Description
Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 µg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Highlights
- Efficient—2 to 10 µg of genomic DNA from 0.2 mL of blood
- Fast—procedure takes approximately 20 minutes
- Universal—purifies genomic DNA from various sources
- Flexible—easy to scale up and down
- Safe—does not involve phenol extraction
Applications
- Purification of high-quality genomic DNA from:
- Whole blood or serum
- Cell culture
- Plant tissues
- Mammalian tissues
- Epithelium samples
- Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol